Home 
Tutorial 
Theory 
References 
oGNM 2.0 
ANM 2.0 
Computational & Systems Biology 
NTHU site

Theory
GAUSSIAN NETWORK MODEL (GNM)
Figure 1: Schematic representation of equilibrium position vectors of residues, their distance vectors and fluctuations.
The GNM is based on the statistical mechanical theory developed by Flory and coworkers for polymer gels (1), where network junctions undergo Gaussian fluctuations. In the GNM, the positions of the network junctions/nodes are identified with the C^{a} atoms, and the elastic springs represent the interactions that stabilize the native contacts. Two residues are assumed to be in contact when their acarbons are separated by less than a cutoff distance R_{c}, which is usually taken around 7 Å. As shown schematically in Figure 1, the fluctuation in the distance between the i^{th} and j^{th} residues is denoted as
ΔR_{ij} = R_{ij}  R_{ij}^{0}^{ }= ΔR_{j }– ΔR_{i} 
(1) 
where R_{ij} is the instantaneous distance vector, R_{ij}^{0} is its equilibrium value and ΔR_{i} = R_{i}  R_{i}^{0} designates the fluctuation in the position vector of residue i. The GNM potential is given by
(2) 
Here G is the Kirchhoff matrix, the offdiagonal elements of which are defined as
Γ_{ij} = 1 if R_{ij }≤ R_{c} Γ_{ij} = 0 if R_{ij }> R_{c} 
(3) 
and the diagonal elements are
Γ_{ii}_{ }=  Σ_{j} Γ_{ij} 
(4) 
where the summation is performed over all offdiagonal elements in the i^{th} row (or column); h(x) is the Heaviside step function equal to 1 if the argument is positive, zero otherwise; ΔR is an Ndimensional vector composed of the fluctuations of the Nresidues.
The crosscorrelations between residue fluctuations are found from the statistical mechanical average
(5) 
where Γ^{1}_{ij} is the ij^{th} element of the pseudoinverse of Γ, V is the GNM potential given by Eq. 2. Therefore the evaluation of crosscorrelations reduces to that of the ijth element of the inverse of Γ.
The determinant of Γ is 0, and hence Γ^{1 }cannot be calculated directly. Instead, it is found from the eigenvalue decomposition Γ = ULU^{T} which results in an ensemble of N1 independent modes. U is the orthogonal matrix whose k^{th} column u_{k} is the k^{th} eigenvector of Γ, and L is the diagonal matrix of eigenvalues l_{k} which are usually organized in ascending order. One of the eigenvalues is identically zero, and the remaining N1 eigenvalues define each the frequency of the N1 GNM modes. The i^{th} element (u_{k})_{i} of u_{k} describes the motion of the i^{th} residue along the k^{th} normal mode coordinate; or, the elements of the k^{th} eigenvector u_{k} represent the distribution of residue displacements (normalized over all residues) along the k^{th} mode axis, and the corresponding eigenvalue l_{k } scales with the frequency of the mode. The size of the motion along the k^{th} mode scales with (1/l_{k})^{1/2}. Thus (1/l_{k})^{1/2} serves as the ‘weight’ of a displacement along mode k. Clearly the lowest frequency modes (small l_{k}) also called slowest modes or softest modes make the largest contribution to the overall motion.
The crosscorrelation between residues can be written as a sum of N1 GNM modes as
(6) 
The meansquare fluctuations of residue i in mode k can be evaluated from Eq. 6 by replacing j by i. The plot of tr[u_{k }u_{k}^{T}] as a function of residue i represents the probability distribution of residue square fluctuations in mode k, also called k^{th} mode profile.
The crosscorrelations are usually shown in a matrix/map format, with the diagonal terms representing the meansquare fluctuations. This ij^{th} element of this matrix, termed crosscorrelation matrix, is
C_{ij }= < ΔR_{i }. ΔR_{j }> 
(7) 
The normalized crosscorrelations are given by
C_{ij }^{(n)}_{ }= < ΔR_{i }. ΔR_{j }> / [< ΔR_{i }. ΔR_{i }> < ΔR_{j }. ΔR_{j }>]^{1/2} 
(8) 
C_{ij }^{(n)}_{ }varies in the range [1, 1] and provides information on orientational correlations between the motions of residues i and j. For more information see references (27) listed below.
Degree of collectivity
The degree of collectivity of a given mode measures the extent to which the structural elements move together in that particular mode. A high degree of collectivity means a highly cooperative mode, that engages a large portion of (if not the entire) structure. Conversely, low collectivity refers to modes that affect small/local regions only. Modes of high degree of collectivity are generally of interest as functionally relevant modes. These are usually found at the low frequency end of the mode spectrum.
Collectivity for a given mode k is a measure of the degree of cooperativity (between residues) in that mode, defined as (8,9)
(9) 
where, k is the mode number, and i is the residue index.
References
1. Flory,P. (1976) Statistical thermodynamics of random networks. Proc. R. Soc. Lond. A, 351, 351380.
2. Bahar,I., Atilgan,A.R. and Erman,B. (1997) Direct evaluation of thermal fluctuations in proteins using a singleparameter harmonic potential. Fold. Des., 2, 173181.
3. Bahar,I., Atilgan,A.R., Demirel,M.C. and Erman,B. (1998) Vibrational Dynamics of Folded Proteins: Significance of Slow and Fast Motions in Relation to Function and Stability. Phys. Rev. Lett., 80, 23.
4. Bahar,I. and Rader,A.J. (2005) Coarsegrained normal mode analysis in structural biology. Curr. Opin. Struct. Biol., 15, 586592.
5. Rader,A.J., Chennubhotla,C., Yang,L.W. and Bahar,I. (2006) The Gaussian network model: Theory and applications. In Bahar,I. and Cui,Q. (eds.), NORMAL MODE ANALYSIS: THEORY AND APPLICATIONS TO BIOLOGICAL AND CHEMICAL SYSTEMS. Chapman & Hall/CRC: Boca Raton, FL, pp. 4164.
6. Eyal,E., Dutta,A. and Bahar,I. (2011) Cooperative dynamics of proteins unraveled by network models. WIREs Comput Mol Sci, 1, 426439.
7. Yang,L.W. (2011) Models with energy penalty on interresidue rotation address insufficiencies of conventional elastic network models. Biophys. J., 100, 17841793.
8. Tama,F. and Sanejouand,Y.H. (2001) Conformational change of proteins arising from normal mode calculations. Protein Eng., 14, 16.
9. Brüschweiler,R. (1995) Collective protein dynamics and nuclear spin relaxation. J. Chem. Phys., 102, 33963403.